猴肾细胞系LLC-MK1、LLC-MK2和LLC-MK2（NCTC-3196）的生长特性及其在病毒研究中的应用。
Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research.
作者信息
HULL R N, CHERRY W R, TRITCH O J
出版信息
J Exp Med. 1962 May 1;115(5):903-18. doi: 10.1084/jem.115.5.903.
Abstract
The establishment of two strains of rhesus monkey kidney cells in continuous tissue culture, the development of a subline adapted to chemically defined medium, and the isolation of several clonal derivatives were described. Growth characteristics, chromosome numbers, malignant potentiality, and freeze storage data are presented. The cells were studied for their sensitivity to a large number of viruses and were extensively compared with primary cultures of monkey kidney cells for sensitivity to poliovirus. The cell strains were not sensitive to all the viruses which could be grown in primary cultures of the same tissue but were susceptible to most of them. In some instances an advantage to the use of the cell strain for certain viruses was noted.
摘要
本文描述了恒河猴肾细胞的两株细胞系在连续组织培养中的建立，一种适应化学成分确定培养基的亚系的培育，以及几种克隆衍生物的分离。文中给出了生长特性、染色体数目、恶性潜能和冷冻保存数据。研究了这些细胞对大量病毒的敏感性，并与猴肾细胞原代培养物对脊髓灰质炎病毒的敏感性进行了广泛比较。这些细胞系对所有能在相同组织原代培养物中生长的病毒并不敏感，但对其中大多数病毒敏感。在某些情况下，注意到使用这些细胞系培养某些病毒具有优势。
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