胰岛素和佛波酯对大鼠脂肪细胞中蛋白激酶C亚型亚细胞分布的影响。

Farese R V, Standaert M L, Francois A J, Ways K, Arnold T P, Hernandez H, Cooper D R

J. A. Haley Veterans' Hospital, Tampa, FL.

Biochem J. 1992 Nov 15;288 ( Pt 1)(Pt 1):319-23. doi: 10.1042/bj2880319.

Effects of insulin and phorbol esters on subcellular distribution of protein kinase C (PKC) isoforms were examined in rat adipocytes. Both agonists provoked rapid decreases in cytosolic, and/or increases in membrane, immunoreactive PKC-alpha, PKC-beta, PKC-gamma, and PKC-epsilon. Effects of phorbol esters on PKC-alpha redistribution to the plasma membrane, however, were much greater than those of insulin. In contrast, insulin, but not phorbol esters, stimulated the translocation of PKC-beta to the plasma membrane, and provoked changes in PKC-zeta redistribution. Neither agonist altered subcellular distribution of PKC-delta, which was detected only in membrane fractions. Our findings indicate that insulin and phorbol esters have overlapping and distinctly different effects on the subcellular redistribution of specific PKC isoforms.

在大鼠脂肪细胞中研究了胰岛素和佛波酯对蛋白激酶C（PKC）亚型亚细胞分布的影响。两种激动剂均引起胞质中免疫反应性PKC-α、PKC-β、PKC-γ和PKC-ε迅速减少，和/或膜中增加。然而，佛波酯对PKC-α重新分布到质膜的作用远大于胰岛素。相反，胰岛素而非佛波酯刺激PKC-β向质膜的转位，并引起PKC-ζ重新分布的变化。两种激动剂均未改变仅在膜组分中检测到的PKC-δ的亚细胞分布。我们的研究结果表明，胰岛素和佛波酯对特定PKC亚型的亚细胞重新分布具有重叠且明显不同的作用。