多巴胺-β-单加氧酶与嗜铬粒膜脂质的相互作用。

Interaction of dopamine-beta-monooxygenase with chromaffin granule membrane lipids.

作者信息

Pogosyan A G, Boyajian A S, Mkrtchyan M Y, Karagezyan K G

机构信息

Institute of Molecular Biology, Academy of Sciences of the Armenian Republic, Yerevan.

出版信息

Biochem Biophys Res Commun. 1992 Oct 30;188(2):678-83. doi: 10.1016/0006-291x(92)91109-4.

DOI:10.1016/0006-291x(92)91109-4

PMID:1445313

Abstract

The interaction between bovine adrenal medullary dopamine-beta-monooxygenase and liposomes from chromaffin granule membrane lipids as a function of pH, lipid and salt concentration was studied by ultracentrifugation. Efficient adsorption of dopamine-beta-monooxygenase to liposomes occurs in the pH range 5.0-6.5 and at low ionic strength. The adsorption was not detected in the case of apoenzyme. The membrane dopamine-beta-monooxygenase forms a complex with liposomes more effective than soluble does. The data obtained lead to certain conclusions about the specificity of complex between the enzyme and liposomes.

摘要

通过超速离心研究了牛肾上腺髓质多巴胺-β-单加氧酶与嗜铬粒膜脂质形成的脂质体之间的相互作用，该相互作用是pH、脂质和盐浓度的函数。多巴胺-β-单加氧酶在pH值5.0 - 6.5范围内和低离子强度下能有效吸附到脂质体上。脱辅基酶的情况下未检测到吸附现象。膜结合的多巴胺-β-单加氧酶与脂质体形成的复合物比可溶性酶形成的复合物更有效。所得数据得出了有关该酶与脂质体之间复合物特异性的某些结论。