Fabri L, Nice E C, Ward L D, Maruta H, Burgess A W, Simpson R J
Ludwig Institute for Cancer Research (Melbourne Branch), Parkville, Victoria, Australia.
Biochem Int. 1992 Oct;28(1):1-9.
The topology of the disulfides in native heparin-binding neurotrophic factor (HBNF) isolated from bovine brain was studied by proteolytic digestion using trypsin, Asp-N endoproteinase and chymotrypsin and peptide mapping. Disulfide-linked peptides were identified by automated Edman degradation. It has been shown that there are disulfide bonds between Cys15-Cys44, Cys23-Cys53, Cys30-Cys57, Cys67-Cys99 and Cys77-Cys109.
