Knox W H, Sax J A, Wilson D R, Sen A K
Can J Physiol Pharmacol. 1977 Oct;55(5):1112-5. doi: 10.1139/y77-153.
The aim of this study was to determine the effect of changes in osmolality on the reduced renal medullary Na-K-ATPase (EC 3.6.1.3) activity of the postobstructive kidney. The effect of osmolality on renal medullary Na-K-ATPase activity was studied by incubating tissue slices from sham-operated and bilaterally obstructed rats in media with osmolality varied before enzyme isolation using sodium chloride, choline chloride, or sucrose. Both sham-operated and bilaterally obstructed rat renal medullary enzyme showed a similar increase in activity with increased osmolality due to sodium chloride. Medullary Na-K-ATPase from the postobstructive kidney also showed increased activity with osmotic changes induced by choline chloride or sucrose. It is proposed that the decrease of Na-K-ATPase activity observed after bilateral ureteral obstruction is due, at least in part, to the loss of the solute concentration gradient in the kidney.
本研究的目的是确定渗透压变化对梗阻后肾脏肾髓质中钠钾ATP酶（EC 3.6.1.3）活性降低的影响。通过将假手术和双侧梗阻大鼠的组织切片在酶分离前用氯化钠、氯化胆碱或蔗糖改变渗透压的培养基中孵育，研究渗透压对肾髓质钠钾ATP酶活性的影响。假手术和双侧梗阻大鼠的肾髓质酶均显示，由于氯化钠导致渗透压升高，酶活性有类似的增加。梗阻后肾脏的髓质钠钾ATP酶在氯化胆碱或蔗糖诱导的渗透压变化下也显示活性增加。有人提出，双侧输尿管梗阻后观察到的钠钾ATP酶活性降低至少部分是由于肾脏中溶质浓度梯度的丧失。
