Hardman P, Spooner B S
Center for Gravitational Studies in Cellular and Developmental Biology, NASA Specialized Center of Research and Training (NSCORT) in Gravitational Biology, Kansas State University, Manhattan.
Int J Dev Biol. 1992 Sep;36(3):423-7.
We examined the biosynthetic patterns of interstitial collagens in mouse embryonic submandibular and sublingual glands cultured in vitro. Rudiments explanted on day 13 of gestation and cultured for 24, 48, and 72 h all synthesized collagen types I, III, and V. However, while the total incorporation of label into collagenous proteins did not change over the three-day culture period, the rate of accumulation of newly synthesized types I and III did change. At 24 h, the ratio of newly synthesized collagen types I:III was approximately 2, whereas at 72 h, the ratio was approximately 5. These data suggest that collagen types I and III may be important in initiation of branching in this organ, but that type I may become dominant in the later stages of development and in maintenance of the adult organ.
我们研究了体外培养的小鼠胚胎下颌下腺和舌下腺中间质胶原的生物合成模式。在妊娠第13天取出的组织原基培养24、48和72小时后，均合成了I型、III型和V型胶原。然而，虽然在三天的培养期内，标记物掺入胶原蛋白质的总量没有变化，但新合成的I型和III型胶原的积累速率确实发生了变化。在24小时时，新合成的I型与III型胶原的比例约为2，而在72小时时，该比例约为5。这些数据表明，I型和III型胶原可能在该器官分支起始过程中很重要，但I型胶原可能在发育后期及成年器官的维持中占主导地位。
