[蒽环类抗生素与DNA结合的定量测定]
[Quantitative determination of anthracycline antibiotic binding with DNA].
作者信息
Bogush T A, Baranov E P, Egudina S V, Tankovich N N
出版信息
Biull Eksp Biol Med. 1992 Jun;113(6):636-8.
PMID:1446037
Abstract
A new method of quantitative intravital assessment of anthracyclines accumulation and binding with DNA in alive cells has been developed. For this purpose DNA specific fluorescent dye Hoechst 33258 was used. Extent of fluorescence quenching of bound with DNA dye correlated with binding of daunomycin with cell DNA after mixing solutions of the drug and cells. It allowed us to determine quantity of drug molecules bound with DNA in the cells during the time.
摘要
已开发出一种对活细胞中蒽环类药物积累及与DNA结合进行定量活体评估的新方法。为此使用了DNA特异性荧光染料Hoechst 33258。在将药物溶液与细胞溶液混合后，与DNA结合的染料的荧光猝灭程度与柔红霉素和细胞DNA的结合相关。这使我们能够在一段时间内确定细胞中与DNA结合的药物分子数量。
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