van Pampus E C, Denkers I A, van Geel B J, Huijgens P C, Zevenbergen A, Ossenkoppele G J, Langenhuijsen M M
Department of Haematology, Free University Hospital, Amsterdam, The Netherlands.
Eur J Haematol. 1992 Sep;49(3):122-7. doi: 10.1111/j.1600-0609.1992.tb00915.x.
There is evidence that mature megakaryocytes migrate into sinusoids, enter the blood and fragment in the vascular bed. We wondered whether differences in expression of adhesion antigens could be associated with the egress of megakaryocytes from bone marrow into the peripheral blood or the fragmentation into platelets. Megakaryocytes from human marrow were purified by counterflow centrifugal elutriation followed by a glycoprotein Ib-dependent agglutination procedure. Megakaryocytes from central venous blood and pulmonary arteries were purified by counterflow centrifugal elutriation alone. Adhesion antigens were labelled in an immunohistochemical assay. Both bone marrow megakaryocytes and platelets from healthy volunteers stained > 75% positive for CD36, CD41, CD42, Cdw49b (alpha subunit VLA2), Cdw49e (alpha subunit VLA5), Cdw49f (alpha subunit VLA6) and CD62. Circulating megakaryocytes, although > 75% positive for CD41, had, unlike platelets and bone marrow megakaryocytes, a reduced and remarkable heterogeneous (5-100% positive) labelling with antibodies against Cdw49b, Cdw49e, Cdw49f. These results could be confirmed by comparing the bone marrow megakaryocytes, circulating megakaryocytes and platelets from 7 patients that were recovered and processed at the same time. Morphologically mature, circulating megakaryocytes have, unlike bone marrow megakaryocytes, a heterogeneous expression of adhesion antigens, especially of Cdw49b, Cdw49e, and Cdw49f.
有证据表明，成熟的巨核细胞迁移至血窦，进入血液，并在血管床中破碎。我们想知道黏附抗原表达的差异是否与巨核细胞从骨髓进入外周血或破碎成血小板有关。通过逆流离心淘析法，随后采用依赖糖蛋白Ib的凝集程序，从人骨髓中纯化巨核细胞。仅通过逆流离心淘析法从中心静脉血和肺动脉中纯化巨核细胞。在免疫组织化学分析中标记黏附抗原。来自健康志愿者的骨髓巨核细胞和血小板对CD36、CD41、CD42、Cdw49b（α亚基VLA2）、Cdw49e（α亚基VLA5）、Cdw49f（α亚基VLA6）和CD62的染色阳性率>75%。循环中的巨核细胞，虽然对CD41的阳性率>75%，但与血小板和骨髓巨核细胞不同，用抗Cdw49b、Cdw49e、Cdw49f抗体标记时，其标记减少且显著异质性（5 - 100%阳性）。通过比较同时回收和处理的7例患者的骨髓巨核细胞、循环巨核细胞和血小板，这些结果可以得到证实。形态学上成熟的循环巨核细胞与骨髓巨核细胞不同，其黏附抗原表达具有异质性，尤其是Cdw49b、Cdw49e和Cdw49f。
