大鼠大脑皮质中Ca(++)-ATP酶的酶超微细胞化学显示

Enzyme ultracytochemical demonstration of Ca(++)-ATPase in the rat cerebral cortex.

作者信息

Zinchuk V S, Tushevsky V F, Bulavka A V

机构信息

Department of Pathological Anatomy, Kiev Medical Institute, USSR.

出版信息

Folia Histochem Cytobiol. 1992;30(1):13-6.

PMID:1446775

Abstract

The localization of Ca(++)-ATPase activity was investigated in the rat cerebral cortex using an ultracytochemical method. Our cytochemical procedure for the detection of enzyme activity is based on an incubation medium consisting of tricine buffer, ATP-disodium salt as substrate, cerium chloride as capturing agent, CaCl2, and levamisole as a nonspecific alkaline phosphatase inhibitor. Final pH was 7.4. Reaction products showing Ca(++)-ATPase activity were localised on the pre- and postsynaptic plasma membrane in association with synaptic vesicles, postsynaptic dendrites and on the axolemma in myelinated nerve fibres. The verification of the main enzymatic properties of Ca(++)-ATPase localization activity is the subject of the following report.

摘要

采用超微细胞化学方法研究了大鼠大脑皮质中Ca(++)-ATP酶活性的定位。我们检测酶活性的细胞化学程序基于一种孵育培养基，其由三羟甲基氨基甲烷缓冲液、作为底物的ATP二钠盐、作为捕获剂的氯化铈、氯化钙以及作为非特异性碱性磷酸酶抑制剂的左旋咪唑组成。最终pH值为7.4。显示Ca(++)-ATP酶活性的反应产物定位于突触前和突触后质膜，与突触小泡、突触后树突相关，以及定位于有髓神经纤维的轴膜上。Ca(++)-ATP酶定位活性主要酶学特性的验证是以下报告的主题。