Wright J R, Polvi S, MacLean H
Department of Pathology, Izaak Walton Killam Children's Hospital, Halifax, Nova Scotia, Canada.
Diabetes. 1992 Dec;41(12):1528-32. doi: 10.2337/diab.41.12.1528.
Certain teleost fish have macroscopically visible islets called BBs that are anatomically discrete. BBs were harvested from Oreochromis nilotica (tilapia) with microscissors, divided, and cultured overnight at 37 degrees C before transplantation into STZ-induced diabetic nude mice. Each mouse received BB fragments from 3-5 fish weighing in aggregate approximately 1.7 kg. Non-FPGs were monitored 5 days/wk. Recipients remained normoglycemic (plasma glucose < 11.1 mM) for 50 days posttransplantation. Mice bearing 50-day-old grafts had essentially normal GTTs. Left nephrectomies then were performed to remove the grafts, and plasma glucose levels in recipient mice rose to > 22.2 mM. Histological examination of graft-bearing kidneys showed viable, vascularized islet tissue containing numerous well-granulated beta-cells; examination of recipient native pancreases revealed small islets composed predominantely of non-beta-cells.
某些硬骨鱼具有肉眼可见的胰岛，称为BB细胞团，它们在解剖学上是离散的。用显微剪刀从尼罗罗非鱼（罗非鱼）中采集BB细胞团，进行分割，并在37℃下过夜培养，然后移植到链脲佐菌素诱导的糖尿病裸鼠体内。每只小鼠接受来自3 - 5条鱼的BB细胞团碎片，这些鱼的总重量约为1.7千克。每周5天监测非空腹血糖（Non-FPG）。移植后50天，受体小鼠保持血糖正常（血浆葡萄糖<11.1 mM）。移植50天的小鼠具有基本正常的葡萄糖耐量试验（GTT）。然后进行左肾切除术以移除移植物，受体小鼠的血浆葡萄糖水平升至>22.2 mM。对带有移植物的肾脏进行组织学检查，发现有存活的、血管化的胰岛组织，其中含有许多颗粒丰富的β细胞；对受体天然胰腺的检查显示，小胰岛主要由非β细胞组成。
