用二硝基苯基 - 聚苯乙烯刺激大鼠肥大细胞的整体脱颗粒作用。

Global degranulation of rat mast cells stimulated with DNP-polystyrene.

作者信息

Sitte I, Stössel H, Kalchschmid G, Romani N, Fritsch P, Varga J M

机构信息

Department of Dermatology, University of Innsbruck, Austria.

出版信息

Immunol Lett. 1992 Jul;33(2):139-43. doi: 10.1016/0165-2478(92)90038-p.

DOI:10.1016/0165-2478(92)90038-p

PMID:1446918

Abstract

Mediator release was studied in rat peritoneal mast cells sensitized with a mouse monoclonal anti-DNP IgE antibody, and stimulated with DNP-ornithine covalently attached to radio-derivatized polystyrene petri dishes. Cells releasing serotonin at maximal rates were investigated by transmission electron microscopy. Generalized exocytosis of granules could be observed, suggesting non-directional release of mediators, and non-compartmentalized action of second messengers in mast cells stimulated with polystyrene-bound DNP. Stimulation of sensitized mast cells by DNP covalently bound to the rigid polystyrene surface is consistent with extrinsic mechanisms proposed for Fc(epsilon)RI receptor action, and suggests that internalization of Fc(epsilon)RI is not needed for triggering cell degranulation.

摘要

在经小鼠单克隆抗二硝基苯酚（DNP）IgE抗体致敏的大鼠腹膜肥大细胞中研究介质释放，并用共价连接到放射性衍生聚苯乙烯培养皿上的DNP-鸟氨酸进行刺激。通过透射电子显微镜研究以最大速率释放血清素的细胞。可以观察到颗粒的普遍胞吐作用，这表明介质的非定向释放以及在用聚苯乙烯结合的DNP刺激的肥大细胞中第二信使的非区室化作用。与刚性聚苯乙烯表面共价结合的DNP对致敏肥大细胞的刺激与针对Fc(ε)RI受体作用提出的外在机制一致，并且表明触发细胞脱颗粒不需要Fc(ε)RI的内化。