Méndez R, Negro A, Martín-Villacorta J
Departamento de Bioquímica y Biología Molecular, Facultades de Biología y Veterinaria, Universidad de León, Spain.
J Chromatogr. 1992 Aug 7;579(1):115-21. doi: 10.1016/0378-4347(92)80369-2.
High-performance liquid chromatographic methods have been developed for the determination of two 6-(1-hydroxyethyl)penems, SCH 29482 (I) and FCE 22101 (II), in serum and urine. Serum samples were combined with an equal volume of methanol to remove proteins and, after centrifugation, an aliquot of the supernatant was analysed by ion-pair chromatography on a reversed-phase C18 column with hexadecyltrimethylammonium bromide as the ion-pairing agent. The compounds were detected by their ultraviolet absorbance at 305 nm for II and 322 nm for I. Urine samples were diluted, filtered and analysed by the same chromatographic procedure. At concentrations of 1-500 micrograms/ml of each compound, the within- and between-day precisions were 1.8-3.6 and 2.6-5.1%, respectively. The detection limit was 0.2 micrograms/ml for I and 0.3 micrograms/ml for II.
已开发出高效液相色谱法用于测定血清和尿液中的两种6-(1-羟乙基)青霉烯类化合物，即SCH 29482（I）和FCE 22101（II）。血清样品与等体积甲醇混合以去除蛋白质，离心后，取上清液的一份子，以十六烷基三甲基溴化铵作为离子对试剂，在反相C18柱上通过离子对色谱法进行分析。化合物通过其在305 nm（II）和322 nm（I）处的紫外吸光度进行检测。尿液样品经稀释、过滤后，采用相同的色谱程序进行分析。在每种化合物浓度为1 - 500微克/毫升时，日内和日间精密度分别为1.8 - 3.6%和2.6 - 5.1%。I的检测限为0.2微克/毫升，II的检测限为0.3微克/毫升。
