Carabias Martinez R, Rodriguez Gonzalo E, Amigo Moran M J, Hernandez Mendez J
Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Salamanca, Spain.
J Chromatogr. 1992 Aug 21;607(1):37-45. doi: 10.1016/0021-9673(92)87052-a.
A sensitive method for the high-performance liquid chromatographic determination of five organophosphorus pesticides (paraoxon, methyl-parathion, ethyl-parathion, guthion and fenitrothion) in fruits and tap and river water samples is described. For the determination of pesticides in fruits a simple and rapid sample preparation procedure was developed that allowed pesticides to be determined at 50-100 micrograms/kg levels with recoveries ranging from 83 to 118% and relative standard deviations below 6%. The determination of pesticide residues in surface water samples was also successfully accomplished. Concentrations at sub-ppb levels can be measured by using a solid-phase concentration step, the recoveries being over 80%. In analyses of both fruits and surface waters, the sensitivity levels achieved were 2-10 times lower than legal limits admitted in the European Economic Community.
描述了一种用于高效液相色谱法测定水果、自来水和河水样品中五种有机磷农药（对氧磷、甲基对硫磷、乙基对硫磷、谷硫磷和杀螟硫磷）的灵敏方法。对于水果中农药的测定，开发了一种简单快速的样品制备程序，该程序能够测定50 - 100微克/千克水平的农药，回收率在83%至118%之间，相对标准偏差低于6%。地表水样品中农药残留的测定也成功完成。通过固相浓缩步骤可以测量亚ppb水平的浓度，回收率超过80%。在水果和地表水的分析中，所达到的灵敏度水平比欧洲经济共同体认可的法定限值低2 - 10倍。
