Shvachko L P, Poiarkova S A, Kostiuchenko N V, Kibirev V K
Ukr Biokhim Zh (1978). 1992 Jul-Aug;64(4):34-7.
Hydrolysis and respective catalytic parameters of hydrolysis of ester peptide substrates that contain residues of hydrophobic and nonpolar amino acids in P2, P3 subsites have been studied. It is shown that efficiency of hydrolysis by thrombin is determined by the length of polypeptide chains and by the nature of the amino acids in P2, P3 subsites in the substrate. In spite of the fact that gamma-thrombin retains the conformation activity of the catalytic centre the local conformation changes of the second binding region of the enzyme have been discovered.
对含有位于P2、P3亚位点的疏水性和非极性氨基酸残基的酯肽底物的水解及其各自的催化参数进行了研究。结果表明，凝血酶的水解效率取决于多肽链的长度以及底物中P2、P3亚位点氨基酸的性质。尽管γ-凝血酶保留了催化中心的构象活性，但已发现该酶第二个结合区域的局部构象发生了变化。
