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F1 - ATP 合酶催化由油酰磷酸和 ADP 合成 ATP。

F1-ATPase-catalyzed synthesis of ATP from oleoylphosphate and ADP.

作者信息

Johnston R, Criddle R S

出版信息

Proc Natl Acad Sci U S A. 1977 Nov;74(11):4919-23. doi: 10.1073/pnas.74.11.4919.

DOI:10.1073/pnas.74.11.4919
PMID:144917
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC432068/
Abstract

Purified preparations of F1-ATPase (ATP phosphohydrolase; EC 3.6.1.3) isolated from yeast mitochondria catalyze the reaction of oleoylphosphate with ADP to yield ATP and oleic acid. Formation of ATP is specifically inhibited by the F1-ATPase inhibitor 1799 and by dinitrophenol. In the presence of F1, dinitrophenol "uncouples" the synthase reaction by causing rapid hydrolysis of oleoylphosphate without ATP formation. It is proposed that this F1 catalyzed ATP synthesis reaction corresponds to the terminal chemical step in oxidative phosphorylation.

摘要

从酵母线粒体中分离得到的纯化的F1 - ATP酶（ATP磷酸水解酶；EC 3.6.1.3）制剂催化油酰磷酸与ADP反应生成ATP和油酸。ATP的形成受到F1 - ATP酶抑制剂1799和二硝基苯酚的特异性抑制。在F1存在的情况下，二硝基苯酚通过导致油酰磷酸快速水解而不形成ATP，从而使合成酶反应“解偶联”。有人提出，这种由F1催化的ATP合成反应对应于氧化磷酸化中的末端化学步骤。

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本文引用的文献

1
On the mode of action of 2, 4-dinitrophenol in uncoupling oxidative phosphorylation.关于2,4-二硝基苯酚在解偶联氧化磷酸化中的作用方式
J Biol Chem. 1958 Dec;233(6):1574-7.
2
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
3
The effect of uncouplers on mitochondria, respiratory enzyme complexes and artificial phospholipid membranes.解偶联剂对线粒体、呼吸酶复合物及人工磷脂膜的作用。
Curr Mod Biol. 1968 May-Jun;2(2):98-105. doi: 10.1016/0303-2647(68)90014-2.
4
Succinyl phosphate and the succinyl coenzyme A synthetase reaction.琥珀酰磷酸与琥珀酰辅酶A合成酶反应。
J Biol Chem. 1969 May 25;244(10):2606-13.
5
Fatty acid mutant of E. coli lacking a beta-hydroxydecanoyl thioester dehydrase.缺乏β-羟基癸酰硫酯脱水酶的大肠杆菌脂肪酸突变体。
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1579-86. doi: 10.1073/pnas.58.4.1579.
6
Characterization and localization of mitochondrial uncoupler binding sites with an uncoupler capable of photoaffinity labeling.利用具有光亲和标记能力的解偶联剂对线粒体解偶联剂结合位点进行表征和定位。
J Biol Chem. 1974 Mar 10;249(5):1356-62.
7
Assembly of the mitochondrial membrane system. I. Characterization of some enzymes of the inner membrane of yeast mitochondria.
J Biol Chem. 1969 Sep 25;244(18):5020-6.
8
Assembly of the mitochondrial membrane system. V. Properties of a dispersed preparation of the rutamycin-sensitive adenosine triphosphatase of yeast mitochondria.线粒体膜系统的组装。V. 酵母线粒体中对鲁塔霉素敏感的三磷酸腺苷酶的分散制剂的性质。
J Biol Chem. 1971 Dec 10;246(23):7328-36.
9
Influence of ring substituents on the action of phenols on some dehydrogenases, phospholinases and the soluble ATPase from mitochondria.
Eur J Biochem. 1971 Aug 16;21(3):416-23. doi: 10.1111/j.1432-1033.1971.tb01486.x.
10
Effectiveness of various unsaturated fatty acids in supporting growth and respiration in Saccharomyces cerevisiae.
J Biol Chem. 1975 Dec 10;250(23):9121-9.