RIS H, PLAUT W
J Cell Biol. 1962 Jun;13(3):383-91. doi: 10.1083/jcb.13.3.383.
The chloroplast of Chlamydomonas moewusii was examined by electron microscopic and cytochemical methods for the possible presence of DNA. Both the Feulgen reaction and acridine orange indicated the presence within the chloroplast of one or more irregularly shaped DNA-containing bodies generally in the vicinity of the pyrenoid. Electron micrographs revealed 25 A microfibrils in these areas which correspond to DNA macromolecules with respect to their location, morphology, and sensitivity to deoxyribonuclease digestion. The possibility that this material is the genetic system of the chloroplast and the hypothesis that the chloroplast represents an evolved endosymbiont are discussed.
采用电子显微镜和细胞化学方法检查了莱茵衣藻的叶绿体,以确定其中是否可能存在DNA。福尔根反应和吖啶橙都表明叶绿体中存在一个或多个形状不规则、含DNA的小体,这些小体通常位于蛋白核附近。电子显微镜照片显示,这些区域有25埃的微纤丝,就其位置、形态和对脱氧核糖核酸酶消化的敏感性而言,它们与DNA大分子相对应。本文讨论了这种物质是叶绿体遗传系统的可能性,以及叶绿体代表一种进化的内共生体的假说。