Edwards J G, Bahl J J, Flink I, Milavetz J, Goldman S, Morkin E
University Heart Center, University of Arizona, Tucson.
Biochem Biophys Res Commun. 1992 Nov 30;189(1):504-10. doi: 10.1016/0006-291x(92)91586-f.
Expression of the human beta-myosin heavy chain (beta MHC) gene was studied by transient assay in culture and in situ by direct injection of plasmids into adult rat hearts. In this report we describe a unique repressor region located -326/-309 (5'-TTGGTGGTCGTGGTCAGT-3') of the human beta MHC gene that is conserved among the rat, rabbit, and human beta MHC genes. This sequence conferred repression onto heterologous promoters when the sequence was located 5' but not 3' to the promoters. This partial positional dependency suggests that the factor may act by limiting the binding of enhancers, located more proximally, to their DNA binding sites.
通过在培养物中进行瞬时分析以及通过将质粒直接注射到成年大鼠心脏中进行原位分析，对人类β-肌球蛋白重链（βMHC）基因的表达进行了研究。在本报告中，我们描述了人类βMHC基因位于-326 / -309（5'-TTGGTGGTCGTGGTCAGT-3'）的一个独特的阻遏区域，该区域在大鼠、兔子和人类的βMHC基因中是保守的。当该序列位于启动子的5'端而非3'端时，此序列赋予了对异源启动子的抑制作用。这种部分位置依赖性表明，该因子可能通过限制更靠近启动子的增强子与其DNA结合位点的结合来发挥作用。
