Roy A C, Ratnam S S
Department of Obstetrics and Gynaecology, National University Hospital, National University of Singapore.
Mol Reprod Dev. 1992 Nov;33(3):303-6. doi: 10.1002/mrd.1080330311.
Five homogenates of human sperm cells were separately incubated with [14C]arachidonic acid in the presence of reduced glutathione, L-tryptophan, and haematin as cofactors. The cyclooxygenase products of arachidonic acid metabolism were extracted, separated, and measured for their radioactivity. The rate of formation of prostaglandin (PG)D2, PGE2, PGF2 alpha, 6-keto PGF1 alpha, and thromboxane (TX)B2 were 18.0 +/- 1.11, 10.9 +/- 0.68, 5.8 +/- 0.21, 3.9 +/- 0.13 and 6.6 +/- 0.52 pmol/10(6) cells/min, respectively. These results are discussed in relation to the hypothesis that cyclooxygenase metabolites of certain polyunsaturated fatty acids play an important part in the sperm acrosome reaction and fertilization.
将五份人类精子细胞匀浆分别与[14C]花生四烯酸在还原型谷胱甘肽、L-色氨酸和血红素作为辅因子的存在下进行孵育。提取、分离花生四烯酸代谢的环氧化酶产物，并测量其放射性。前列腺素（PG）D2、PGE2、PGF2α、6-酮-PGF1α和血栓素（TX）B2的生成速率分别为18.0±1.11、10.9±0.68、5.8±0.21、3.9±0.13和6.6±0.52 pmol/10(6)个细胞/分钟。结合某些多不饱和脂肪酸的环氧化酶代谢产物在精子顶体反应和受精中起重要作用这一假说对这些结果进行了讨论。
