Sellmayer A, Weber P C
Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Universität München, FRG.
Eicosanoids. 1992;5 Suppl:S51-2.
Experiments were performed in rat mesangial cells indicating that endogenous non-cyclooxygenase metabolites of AA act to modulate the mitogenic response. Irrespective of the mitogen used, metabolism of AA by LO and/or CP 450 influences induction of cell growth and expression of the immediate early response genes c-fos and Egr-1. In accordance with other reports (3,4) on the influence of eicosanoids on gene expression, our observations indicate that endogenous eicosanoids may act as intracellular mediators. In the mitogenic response, at least two mechanisms of modulation seem possible: i) AA and its metabolites may act by modulating other 2nd messenger systems or ii) eicosanoids may directly regulate the expression of growth-related genes, e.g. by interacting with a fatty acid response element in the promoter region of respective genes.
在大鼠系膜细胞中进行的实验表明，花生四烯酸（AA）的内源性非环氧化酶代谢产物可调节有丝分裂原反应。无论使用何种有丝分裂原，AA通过脂氧合酶（LO）和/或细胞色素P450（CP 450）的代谢都会影响细胞生长的诱导以及即刻早期反应基因c-fos和Egr-1的表达。与其他关于类花生酸对基因表达影响的报道（3,4）一致，我们的观察结果表明内源性类花生酸可能作为细胞内介质起作用。在有丝分裂原反应中，至少有两种调节机制似乎是可能的：i）AA及其代谢产物可能通过调节其他第二信使系统起作用，或者ii）类花生酸可能直接调节生长相关基因的表达，例如通过与各个基因启动子区域中的脂肪酸反应元件相互作用。
