Flemming Peer, Tillmann Hans L, Barg-Hock Hannelore, Kleeberger Wolfram, Manns Michael P, Klempnauer Juergen, Kreipe Hans H
Institut für Pathologie, Institut fuer Pathologie, Medizinische Hochschule Hannover, Germany. Flemming.Peer@ mh-hannover.de
Transplantation. 2003 Sep 15;76(5):871-3. doi: 10.1097/01.TP.0000082819.85398.59.
Hepatocellular carcinomas (HCC) that originate de novo in liver transplants without preceding HCC in the explanted organ have only rarely been reported. Because recent data demonstrated a mixed hepatocellular chimerism caused by the integration of host-derived stem cells, a study was conducted on the origin of tumor cells in de novo HCC.
From two cases of de novo HCC arising in liver transplants after hepatitis B reinfection, tumor cells and non-neoplastic liver cells from the patient's own liver and donor liver were isolated by laser microdissection, and highly polymorphic short tandem DNA repeats (STR) were investigated.
Isolated tumor cells revealed donor-specific STR genotypes that could clearly be discriminated from the genotype of the host.
Hepatitis B virus-associated de novo HCC in liver transplants is of donor but not of host origin. The new technique described here can also discriminate between true recurrence of the original tumor and new recipient tumors.
肝移植中无移植前肝癌的肝脏发生的原发性肝细胞癌（HCC）鲜有报道。由于最近的数据表明宿主来源干细胞整合导致混合性肝细胞嵌合现象，因此开展了一项关于原发性肝癌肿瘤细胞起源的研究。
从两例乙肝再感染后肝移植出现的原发性肝癌病例中，通过激光显微切割分离患者自身肝脏和供体肝脏的肿瘤细胞及非肿瘤性肝细胞，并研究高度多态性的短串联DNA重复序列（STR）。
分离出的肿瘤细胞显示出供体特异性STR基因型，可与宿主基因型明显区分。
肝移植中与乙肝病毒相关的原发性肝癌起源于供体而非宿主。本文所述的新技术还可区分原发肿瘤的真正复发与新的受体肿瘤。
