Tandy N P, Seghatchian M J, Bessos H
South-western Regional Transfusion Centre, Bristol, UK.
Blood Coagul Fibrinolysis. 1992 Oct;3(5):625-8. doi: 10.1097/00001721-199210000-00016.
In view of transfusion reactions and alloimmunization associated with leucocyte contamination of platelet concentrates (PC), there is a general move towards the production of leuco-poor PC. This goal is currently pursued by the production of various PC using buffy coat and apheresis techniques. Although there is no overall consensus on the meaning of 'leuco-poor', by assuming that this refers to a level of 5-50 x 10(7) leucocytes per PC, we were able to make comparisons with available systems used in Europe. In addition to platelet and white cell counts, other markers of PC quality were assessed in some cases. These included traditional markers (such as hypotonic stress response, pH, and lactate and beta-thromboglobulin levels) and newer markers (such as glycocalicin and plasma von Willebrand factor levels). Our preliminary results showed appreciable differences in platelet and white cell content of PC prepared by various types of apheresis equipment. Appreciable differences in the quality of stored PC were also observed between routine PC (non-leuco-poor and buffy coat and apheresed PC (leuco-poor).
鉴于与血小板浓缩物（PC）白细胞污染相关的输血反应和同种免疫，目前普遍倾向于生产白细胞含量低的PC。目前通过使用血沉棕黄层和单采技术生产各种PC来实现这一目标。尽管对于“白细胞含量低”的含义尚无总体共识，但假设其指的是每个PC中白细胞水平为5 - 50×10⁷，我们能够与欧洲使用的现有系统进行比较。除了血小板和白细胞计数外，在某些情况下还评估了PC质量的其他指标。这些指标包括传统指标（如低渗应激反应、pH值、乳酸和β - 血小板球蛋白水平）和新指标（如糖萼蛋白和血浆血管性血友病因子水平）。我们的初步结果显示，通过各种类型的单采设备制备的PC在血小板和白细胞含量上存在明显差异。在常规PC（非白细胞含量低的）与血沉棕黄层PC和单采PC（白细胞含量低的）之间，也观察到储存PC质量存在明显差异。
