Boll I T, Domeyer C, Bührer C
Department of Medicine II, Neukölln Hospital, Berlin, Federal Republic of Germany.
Blood Cells. 1992;18(2):267-77; discussion 278-9.
Using high-resolution phase contrast time-lapse microcinematography, slow movements (0.8-2.0 microns/minute) of human myeloblasts, monoblasts, and megakaryocytes can be recorded. Upon maturation to promyelocytes, motility is lost until cells have reached the stage of metamyelocytes (0.4 microns/minute). Motility increases sharply following maturation into segmented neutrophils (20.4 microns/minute). Monocytes and promonocytes display a mean track velocity of 7.1 microns/minute. The distribution of lymphocyte velocities is not bell-shaped but shows three maxima of 2.1 microns/minute, 7.8 microns/minute, and 18.4 microns/minute. Atypical lymphocytes from patients with infectious mononucleosis belong to the fast group, whereas lymphocytes activated in vitro by mitogens belong to the slow group. Red blood cell precursors from normal human bone marrow do not move actively. In contrast, erythroleukemic blasts show a motility comparable to normal myeloblasts. Similarly, acute promyelocytic leukemia cells move at 6.7 microns/minute, while their normal counterparts are sessile. Increased motility is also observed in blast cells from a variety of acute myelogenous and lymphoblastic leukemias.
利用高分辨率相差延时显微电影摄影术，能够记录人类成髓细胞、原单核细胞和巨核细胞的缓慢运动（0.8 - 2.0微米/分钟）。成熟为早幼粒细胞后，细胞失去运动能力，直至达到晚幼粒细胞阶段（0.4微米/分钟）。成熟为分叶核中性粒细胞后，运动能力急剧增加（20.4微米/分钟）。单核细胞和前单核细胞的平均轨迹速度为7.1微米/分钟。淋巴细胞速度的分布并非钟形，而是呈现出2.1微米/分钟、7.8微米/分钟和18.4微米/分钟三个峰值。传染性单核细胞增多症患者的非典型淋巴细胞属于快速组，而体外被有丝分裂原激活的淋巴细胞属于慢速组。正常人骨髓中的红细胞前体不活跃移动。相比之下，红白血病母细胞的运动能力与正常成髓细胞相当。同样，急性早幼粒细胞白血病细胞以6.7微米/分钟的速度移动，而其正常对应细胞则静止不动。在各种急性髓系白血病和淋巴细胞白血病的母细胞中也观察到运动能力增加。
