奇异变形杆菌血清学独立菌株TG 332的O-多糖结构，该菌株来自新提议的变形杆菌血清群O50。

Structure of the O-polysaccharide of a serologically separate strain of Proteus mirabilis, TG 332, from a new proposed Proteus serogroup O50.

作者信息

Kołodziejska Katarzyna, Kondakova Anna N, Zych Krystyna, Senchenkova Sof'ya N, Shashkov Alexander S, Knirel Yuriy A, Sidorczyk Zygmunt

机构信息

Department of General Microbiology, Institute Microbiology and Immunology, University of Łódz, Banacha 12/16, 90-237 Łódz, Poland.

出版信息

Carbohydr Res. 2003 Sep 26;338(20):2105-9. doi: 10.1016/s0008-6215(03)00350-1.

DOI:10.1016/s0008-6215(03)00350-1

PMID:14505878

Abstract

The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Proteus mirabilis TG 332 strain. The following structure of the O-polysaccharide was determined by chemical methods along with NMR spectroscopy, including 2D COSY, TOCSY, ROESY and 1H, 13C HMQC experiments: [see equation in text]. The O-polysaccharide studied has a unique structure among Proteus O-antigens. Accordingly, P. mirabilis TG 332 is serologically separate, and we propose to classify this strain into a new Proteus serogroup, O50. The nature of minor epitopes that provide a cross-reactivity of P. mirabilis TG 332 O-antiserum with the LPS of P. mirabilis O30 and Proteus penneri 34 (O60) is discussed.

摘要

通过温和酸降解奇异变形杆菌TG 332菌株的脂多糖获得了O-多糖。采用化学方法结合核磁共振波谱法（包括二维COSY、TOCSY、ROESY以及1H、13C HMQC实验）确定了该O-多糖的以下结构：[见文中方程式]。所研究的O-多糖在变形杆菌O抗原中具有独特结构。因此，奇异变形杆菌TG 332在血清学上是独立的，我们建议将该菌株归类为一个新的变形杆菌血清群，即O50。文中讨论了使奇异变形杆菌TG 332抗血清与奇异变形杆菌O30和彭氏变形杆菌34（O60）的脂多糖产生交叉反应的次要表位的性质。