Zych Krystyna, Perepelov Andrei, Baranowska Agata, Zabłotni Agnieszka, Shashkov Alexander S, Knirel Yuriy A, Sidorczyk Zygmunt
Department of General Microbiology, Institute of Microbiology and Immunology, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
FEMS Immunol Med Microbiol. 2005 Mar 1;43(3):351-6. doi: 10.1016/j.femsim.2004.09.004.
An alkali-treated lipopolysaccharide of Proteus penneri strain 60 was studied by chemical analyses and 1H, 13C and 31P NMR spectroscopy, and the following structure of the linear pentasaccharide-phosphate repeating unit of the O-polysaccharide was established: 6)-alpha-D-Galp-(1-->3)-alpha-L-FucpNAc-(1-->3)-alpha-D-GlcpNAc-(1-->3)-beta-D-Quip4NAc-(1-->6)-alpha-D-Glcp-1-P-(O--> Rabbit polyclonal O-antiserum against P. penneri 60 reacted with both core and O-polysaccharide moieties of the homologous LPS. Based on the unique O-polysaccharide structure and serological data, we propose to classify P. penneri 60 into a new, separate Proteus serogroup O70. A weak cross-reactivity of P. penneri 60 O-antiserum with the lipopolysaccharide of Proteus vulgaris O8, O15 and O19 was observed and discussed in view of the chemical structures of the O-polysaccharides.
通过化学分析以及¹H、¹³C和³¹P核磁共振光谱对彭氏变形杆菌60株经碱处理的脂多糖进行了研究，确定了O-多糖线性五糖磷酸重复单元的以下结构：6)-α-D-吡喃半乳糖-(1→3)-α-L-岩藻糖胺-(1→3)-α-D-吡喃葡萄糖胺-(1→3)-β-D-喹诺糖4-胺-(1→6)-α-D-吡喃葡萄糖-1-P-(O→ 兔抗彭氏变形杆菌60的多克隆O抗血清与同源脂多糖的核心和O-多糖部分均发生反应。基于独特的O-多糖结构和血清学数据，我们建议将彭氏变形杆菌60归类为一个新的、独立的变形杆菌血清群O70。鉴于O-多糖的化学结构，观察并讨论了彭氏变形杆菌60 O抗血清与普通变形杆菌O8、O15和O19的脂多糖之间的弱交叉反应性。
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