潘氏变形杆菌60型O-多糖结构及脂多糖血清学研究，该菌被归类为一个新的变形杆菌血清群O70。
Structure of the O-polysaccharide and serological studies of the lipopolysaccharide of Proteus penneri 60 classified into a new Proteus serogroup O70.
作者信息
Zych Krystyna, Perepelov Andrei, Baranowska Agata, Zabłotni Agnieszka, Shashkov Alexander S, Knirel Yuriy A, Sidorczyk Zygmunt
机构信息
Department of General Microbiology, Institute of Microbiology and Immunology, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland.
出版信息
FEMS Immunol Med Microbiol. 2005 Mar 1;43(3):351-6. doi: 10.1016/j.femsim.2004.09.004.
An alkali-treated lipopolysaccharide of Proteus penneri strain 60 was studied by chemical analyses and 1H, 13C and 31P NMR spectroscopy, and the following structure of the linear pentasaccharide-phosphate repeating unit of the O-polysaccharide was established: 6)-alpha-D-Galp-(1-->3)-alpha-L-FucpNAc-(1-->3)-alpha-D-GlcpNAc-(1-->3)-beta-D-Quip4NAc-(1-->6)-alpha-D-Glcp-1-P-(O--> Rabbit polyclonal O-antiserum against P. penneri 60 reacted with both core and O-polysaccharide moieties of the homologous LPS. Based on the unique O-polysaccharide structure and serological data, we propose to classify P. penneri 60 into a new, separate Proteus serogroup O70. A weak cross-reactivity of P. penneri 60 O-antiserum with the lipopolysaccharide of Proteus vulgaris O8, O15 and O19 was observed and discussed in view of the chemical structures of the O-polysaccharides.
通过化学分析以及¹H、¹³C和³¹P核磁共振光谱对彭氏变形杆菌60株经碱处理的脂多糖进行了研究，确定了O-多糖线性五糖磷酸重复单元的以下结构：6)-α-D-吡喃半乳糖-(1→3)-α-L-岩藻糖胺-(1→3)-α-D-吡喃葡萄糖胺-(1→3)-β-D-喹诺糖4-胺-(1→6)-α-D-吡喃葡萄糖-1-P-(O→ 兔抗彭氏变形杆菌60的多克隆O抗血清与同源脂多糖的核心和O-多糖部分均发生反应。基于独特的O-多糖结构和血清学数据，我们建议将彭氏变形杆菌60归类为一个新的、独立的变形杆菌血清群O70。鉴于O-多糖的化学结构，观察并讨论了彭氏变形杆菌60 O抗血清与普通变形杆菌O8、O15和O19的脂多糖之间的弱交叉反应性。
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