通过尺寸排阻色谱法测量蛋白质-蛋白质相互作用。
Measurements of protein-protein interactions by size exclusion chromatography.
作者信息
Bloustine J, Berejnov V, Fraden S
机构信息
Complex Fluids Group, The Martin J. Fisher School of Physics, Brandeis University, Waltham, Massachusetts 02454, USA.
出版信息
Biophys J. 2003 Oct;85(4):2619-23. doi: 10.1016/S0006-3495(03)74684-0.
Abstract
A method is presented for determining second virial coefficients (B(2)) of protein solutions from retention time measurements in size exclusion chromatography. We determine B(2) by analyzing the concentration dependence of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B(2) from positive to negative values in lysozyme and bovine serum albumin solutions. Our size exclusion chromatography results agree quantitatively with data obtained by light scattering.
摘要
本文提出了一种通过尺寸排阻色谱法中的保留时间测量来测定蛋白质溶液第二维里系数（B(2)）的方法。我们通过分析色谱分配系数的浓度依赖性来确定B(2)。我们展示了该方法追踪溶菌酶和牛血清白蛋白溶液中B(2)从正值到负值变化的能力。我们的尺寸排阻色谱结果与通过光散射获得的数据在数量上一致。
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