Dinno M A, Ando M, Dinno F H, Huang K C, Rehm W S
Am J Physiol. 1977 Mar;232(3):E251-7. doi: 10.1152/ajpendo.1977.232.3.E251.
In vitro studies on H+ secretion, potential difference (PD), short-circuit current (Isc), and resistance across stripped mucosa of frog stomach in Cl-medium have shown that addition of nicotine in the serum bathing fluid caused a marked inhibition of the H+ secretory rate and an increase of PD and Isc without change of the transmucosal resistance. A dose-response correlation was indicated. During the first 8 min, the changes in the measured parameters, namely, PD versus Ih and Isc versus Ih, were linear. After 8 min, a deviation from linearity was observed. From the slope of the regression lines, the resistance of the electrogenic Cl- pump on the mucosal membrane (Rcl) was calculated to be 127 omega cm2 and the resistance of the chloride pathway on the serosal side (Rcl) was 407 omega cm2. The resistance of the H+ pump on the mucosal membrane (Rh) in Cl- medium was estimated to be 385 omega cm2. The sum of the emf's of the Cl+ pump on the mucosal membrane and of the Cl- gradient across the serosal membrane, namely Ecl + Ecl, was found to be 35 mV. The presence of such linear relationships between measured versus the H+ rate and Isc versus Ih lends support to the electrogenic theory of HCl secretion.
在氯离子培养基中对青蛙胃剥离黏膜的氢离子分泌、电位差（PD）、短路电流（Isc）及跨膜电阻进行的体外研究表明，在浴液中添加尼古丁会显著抑制氢离子分泌速率，使电位差和短路电流增加，而跨膜电阻不变。呈现出剂量 - 反应相关性。在最初的8分钟内，所测参数的变化，即电位差与氢离子分泌速率（Ih）以及短路电流与氢离子分泌速率的关系呈线性。8分钟后，观察到偏离线性关系。根据回归线的斜率，计算出黏膜膜上生电氯离子泵的电阻（Rcl）为127Ω·cm²，浆膜侧氯离子途径的电阻（Rcl）为407Ω·cm²。氯离子培养基中黏膜膜上氢离子泵的电阻（Rh）估计为385Ω·cm²。发现黏膜膜上氯离子泵的电动势与跨浆膜氯离子梯度的电动势之和，即Ecl + Ecl为35mV。所测参数与氢离子分泌速率以及短路电流与氢离子分泌速率之间存在这种线性关系，支持了盐酸分泌的生电理论。
