Sawane M V, Kaore S B, Gaikwad R D, Patil P M, Patankar S S, Deshkar A M
Department of Physiology, Government Medical College, Nagpur-440003.
Indian J Med Sci. 2002 Nov;56(11):560-6.
Lactate dehydrogenase isoenzyme patterns were obtained in the seman of 93 male partners of infertile couples and 28 proven fertile subjects as a control group. Sperm mitochondrial activity index (SMAI) alongwith the conventional seminal parameters was studied for all the subjects excepting the azoospermic and vasectomised males. Only LDH-C4, a germ cell specific isoenzyme activity varied with the variation in sperm density. LDH-C4 activity varied significantly (p<0.001) within and between different groups. Lactate dehydrogenase-C4 activity was absent in 17 azoospermic samples, confirming its germinal epithelial origin, as well as in 8 samples of vasectomised males. In one azoospermic sample, there were many immature germ cells along with surprisingly high LDH-C4 activity suggesting more activity of germinal epithelium associated with high LDH-C4 activity. LDH-C4 activity was reduced significantly in oligozoospermic samples in proportion to sperm density, thus confirming strong correlation (p<0.001) between LDH-C4 and sperm density. There was statistically significant correlation between LDH-C4 and percentage sperm motility as well as between LDH-C4 and Sperm Mitochondrial Activity Index (SMAI) (probability varying from p<0.05 to p<0.01 in different groups), but no such correlation was found between LDH-C4 and sperm morphology. The data confirms LDH-C4 as a germinal epithelial marker. Its relationship with percentage sperm motility is suggestive of definite role of LDH-C4 in evaluation of the spermatozoal quality, similarly its relationship with Sperm Mitochondrial Activity Index (SMAI score) suggest the role of LDH-C4 in metabolism of the spermatocytes and sperms, though further studies are required for clear and detailed understanding of its metabolic role in semen.
对93名不育夫妇男性伴侣的精液以及28名经证实可育的受试者精液作为对照组进行乳酸脱氢酶同工酶谱分析。除无精子症和输精管结扎男性外，对所有受试者研究了精子线粒体活性指数（SMAI）以及传统精液参数。只有生殖细胞特异性同工酶LDH - C4的活性随精子密度变化而变化。不同组内和组间LDH - C4活性差异显著（p<0.001）。17份无精子症样本以及8份输精管结扎男性样本中均未检测到乳酸脱氢酶 - C4活性，证实了其生精上皮起源。在一份无精子症样本中，存在许多未成熟生殖细胞，同时LDH - C4活性出奇地高，这表明生精上皮活性增强与高LDH - C4活性相关。少精子症样本中LDH - C4活性随精子密度显著降低，从而证实LDH - C4与精子密度之间存在强相关性（p<0.001）。LDH - C4与精子活力百分比以及LDH - C4与精子线粒体活性指数（SMAI）之间存在统计学显著相关性（不同组概率从p<0.05到p<0.01不等），但未发现LDH - C4与精子形态之间存在此类相关性。数据证实LDH - C4为生精上皮标志物。其与精子活力百分比的关系表明LDH - C4在评估精子质量方面具有明确作用，同样，其与精子线粒体活性指数（SMAI评分）的关系表明LDH - C4在精母细胞和精子代谢中的作用，不过要清晰详细地了解其在精液中的代谢作用还需要进一步研究。
