Yamayoshi Asako, Kato Kiyoko, Iwase Reiko, Yamaoka Tetsuji, Wake Norio, Murakami Akira
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Kyoto 606-8585, Japan.
Nucleic Acids Res Suppl. 2003(3):75-6. doi: 10.1093/nass/3.1.75.
To reinforce antisense effects, we focused on the photo-cross-linking ability of psoralen derivatives and examined the applicability of the photodynamic antisense therapy to cancer cells using psoralen-conjugated oligo(nucleosides phosphorothioate)s (Ps-S-Oligo). The regulatory effects of Ps-S-Oligos on the proliferation of cervical carcinoma were evaluated. The inhibitory effects were found to be significant (IC50=16 nM) without any drug delivery systems, and it was also found that the mechanism was associated with the p53 induced apoptosis. This result suggests that the photodynamic antisense therapy will be a promising concept for the cancer therapy.
为增强反义效应，我们聚焦于补骨脂素衍生物的光交联能力，并使用补骨脂素偶联的寡聚（硫代磷酸核苷）（Ps-S-寡聚体）研究了光动力反义疗法对癌细胞的适用性。评估了Ps-S-寡聚体对子宫颈癌增殖的调节作用。发现其在没有任何药物递送系统的情况下具有显著的抑制作用（IC50 = 16 nM），并且还发现其作用机制与p53诱导的细胞凋亡有关。该结果表明光动力反义疗法将是癌症治疗的一个有前景的概念。
