通过损伤特异性和细胞活性评估hSMUG1的修复作用。

Repair roles of hSMUG1 assessed by damage specificity and cellular activity.

作者信息

Masaoka Aya, Matsubara Mayumi, Tanaka Tamon, Terato Hiroaki, Ohyama Yoshihiko, Kubo Kihei, Ide Hiroshi

机构信息

Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai 599-8531, Japan.

出版信息

Nucleic Acids Res Suppl. 2003(3):263-4. doi: 10.1093/nass/3.1.263.

DOI:10.1093/nass/3.1.263

PMID:14510481

Abstract

Single-strand-selective monofunctional uracil-DNA glycosylase (SMUG1) was previously identified as a putative backup enzyme of major mammalian uracil-DNA glycosylase (UDG). However, the subsequent studies have shown conflicting results about the substrate specificity of SMUG1. In the present study, to clarify the repair role of SMUG1, we determined the damage specificity of purified human SMUG1 (hSMUG1) and its contribution to repair of oxidized bases in HeLa cell extracts.

摘要

单链选择性单功能尿嘧啶-DNA糖基化酶（SMUG1）先前被鉴定为主要哺乳动物尿嘧啶-DNA糖基化酶（UDG）的一种假定备用酶。然而，随后的研究关于SMUG1的底物特异性给出了相互矛盾的结果。在本研究中，为了阐明SMUG1的修复作用，我们确定了纯化的人SMUG1（hSMUG1）的损伤特异性及其对HeLa细胞提取物中氧化碱基修复的贡献。