DNA特定结构元件在与修复酶尿嘧啶-DNA糖基化酶相互作用中的作用。
Role of DNA definite structural elements in interaction with repair enzyme uracil-DNA glycosylase.
作者信息
Kubareva E A, Vasilenko N L, Vorobjeva O V, Volkov E M, Oretskaya T S, Korshunova G A, Nevinsky G A
机构信息
A. N. Belozersky Institute of Physical and Chemical Biology, Chemistry Department, Moscow State University, Russia.
出版信息
Biochem Mol Biol Int. 1998 Oct;46(3):597-606. doi: 10.1080/15216549800204122.
Interaction of different oligodeoxyribonucleotides (oligos), their analogs and oligonucleopeptide with uracil-DNA glycosylase (UDG) from human placenta was investigated. It is shown that there is no considerable contribution of heterocyclic bases of DNA to UDG-substrate binding but the UDG interaction with some DNA phosphate groups is necessary for enzyme-substrate recognition. However the phosphate group adjacent to single dU from the 3'-end in oligo is not involved into the electrostatic contact with UDG. It is found that UDG has the high affinity to its reaction product. An oligonucleotide containing a single 2'-deoxy-2'-aminouridine is a non-hydrolyzable substrate analog for UDG.
研究了不同的寡脱氧核糖核苷酸（oligos）、其类似物和寡核苷酸肽与人胎盘尿嘧啶-DNA糖基化酶（UDG）的相互作用。结果表明，DNA的杂环碱基对UDG-底物结合没有显著贡献，但UDG与一些DNA磷酸基团的相互作用是酶-底物识别所必需的。然而，寡聚物中3'-末端单个dU相邻的磷酸基团不参与与UDG的静电接触。发现UDG对其反应产物具有高亲和力。含有单个2'-脱氧-2'-氨基尿苷的寡核苷酸是UDG的不可水解底物类似物。
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