用中性糖簇纳米颗粒包裹DNA。迈向人工病毒的一步。

Encapsulation of DNA with neutral glycocluster nanoparticles. A step toward artificial viruses.

作者信息

Sando Shinsuke, Sasaki Toshinori, Aoyama Yasuhiro

机构信息

Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto 606-8501, Japan.

出版信息

Nucleic Acids Res Suppl. 2003(3):289-90. doi: 10.1093/nass/3.1.289.

DOI:10.1093/nass/3.1.289

PMID:14510494

Abstract

Number and size-controlled macromolecular associations are common in biology with viruses as a typical example. We report here a novel example of artificial viruses, in which the double-helical DNA is coated with 4-nm sized neutral glycocluster nanoparticles (GNPs). The resulting "glycoviruses" are compactly-packed, well charge-shielded (zeta approximately = 0 mV), and effectively transfect cell cultures without notable cytotoxicity.

摘要

数量和大小可控的大分子聚集体在生物学中很常见，病毒就是一个典型例子。我们在此报告一种人工病毒的新例子，其中双螺旋DNA被4纳米大小的中性糖簇纳米颗粒（GNP）包裹。由此产生的“糖病毒”紧密堆积，电荷屏蔽良好（ζ电位约为0 mV），并能有效转染细胞培养物，且无明显细胞毒性。