Cloning and expression of bglF gene from alkaliphilic Nocardiopsis sp. strain F96.
作者信息
Masuda Sumiko, Endo Kimiko, Hayami Tokusuke, Fukazawa Tetsuya, Yatsunami Rie, Nakamura Satoshi
机构信息
Department of Bioengineering, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
出版信息
Nucleic Acids Res Suppl. 2003(3):317-8. doi: 10.1093/nass/3.1.317.
PMID:14510508
Abstract
The gene encoding a novel beta-1,3-glucanase was cloned from alkaliphilic Nocardiopsis sp. F96 and sequenced. The gene contained an open reading frame of 936 bp. The deduced amino acid sequence of the beta-1,3-glucanase exhibited highest homology to those of family 16 glucanases, suggesting that the enzyme belonged to family 16. The beta-1,3-glucanase gene was functionally expressed in Escherichia coli.
摘要
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