Veronese Rodrigues Maria de Lourdes, de Castro Figueiredo José Fernando, Deghaide Neifi Hassan Saloum, Romão Erasmo, Vieira de Souza Nivaldo, Donadi Eduardo Antonio
Faculty of Medicine of Ribeirão Preto, Ophthalmology 12 andar, Campus USP, University of São Paulo, 14048-900 Ribeirão Preto, São Paulo, Brazil.
Acta Ophthalmol Scand. 2003 Oct;81(5):514-6. doi: 10.1034/j.1600-0420.2003.00098.x.
To evaluate the human leucocyte antigen (HLA) class 1 (HLA-A and HLA-B) and HLA class 2 (HLA-DRB1 and HLA-DQB1) allele profiles in the susceptibility to cytomegalovirus retinitis (CMV-R) in patients with AIDS.
Cytomegalovirus retinitis was clinically diagnosed by indirect binocular ophthalmoscopy. Human leucocyte antigens class 1 were typed using a complement-dependent microlymphocytotoxicity assay, and HLA class 2 alleles were identified using amplified DNA hybridized to sequence-specific oligonucleotide primers.
The frequencies of HLA class 1 antigens and HLA class 2 alleles observed in patients and controls were similar; however, HLA-A31 antigen was over-represented in patients with AIDS, independent of the presence of CMV-R.
There was no association between HLA molecules/alleles and CMV-R in Brazilian patients with AIDS. However, the results support the role of the HLA system in the susceptibility to developing AIDS.
评估人类白细胞抗原（HLA）I类（HLA - A和HLA - B）及HLA II类（HLA - DRB1和HLA - DQB1）等位基因谱在艾滋病患者巨细胞病毒性视网膜炎（CMV - R）易感性中的作用。
通过间接双眼检眼镜进行巨细胞病毒性视网膜炎的临床诊断。使用补体依赖的微量淋巴细胞毒性试验对HLA I类抗原进行分型，使用与序列特异性寡核苷酸引物杂交的扩增DNA鉴定HLA II类等位基因。
患者和对照组中观察到的HLA I类抗原和HLA II类等位基因频率相似；然而，HLA - A31抗原在艾滋病患者中过度表达，与CMV - R的存在无关。
在巴西艾滋病患者中，HLA分子/等位基因与CMV - R之间无关联。然而，结果支持HLA系统在艾滋病易感性中的作用。
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