在部分健康中国受试者及早发型2型糖尿病患者中，两种载脂蛋白A-I基因MspI多态性与高密度脂蛋白（HDL）胆固醇水平及肥胖指标的关联

Association of two apolipoprotein A-I gene MspI polymorphisms with high density lipoprotein (HDL)-cholesterol levels and indices of obesity in selected healthy Chinese subjects and in patients with early-onset type 2 diabetes.

作者信息

Ma Yan Qing, Thomas G Neil, Ng Maggie C Y, Critchley Julian A J H, Cockram Clive S, Chan Juliana C N, Tomlinson Brian

机构信息

Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong SAR.

出版信息

Clin Endocrinol (Oxf). 2003 Oct;59(4):442-9. doi: 10.1046/j.1365-2265.2003.01865.x.

DOI:10.1046/j.1365-2265.2003.01865.x

PMID:14510906

Abstract

OBJECTIVE

Previous studies have reported associations between two apolipoprotein A-I (apoA-I) gene MspI polymorphisms (G-75A and C83T) and high density lipoprotein (HDL)-cholesterol and/or apoA-I levels, but have not investigated the relationship with obesity.

METHODS

We determined the distribution of these polymorphisms in 482 early-onset (< or = 40 years) Type 2 Chinese diabetics and 167 Chinese selected healthy controls.

RESULTS

The -75A and 83T allele frequencies were similar in the diabetic and healthy subjects. In the healthy control subjects, HDL-cholesterol levels were significantly higher in the AA homozygotes than in the GG/GA carriers (1.74 +/- 0.58 vs. 1.45 +/- 0.58 mmol/l, P<0.001). Furthermore, analyses showed a significant relationship between increasing HDL-cholesterol tertiles and the AA genotype frequency in the selected healthy subjects (3.6, 8.9 and 16.1%, P=0.026). For the C83T polymorphism, healthy male CT carriers had higher HDL-cholesterol levels than CC homozygotes (1.71 +/- 0.57 vs. 1.25 +/- 0.30 mmol/l, P=0.001), but this was not found in females. No relationship between these polymorphisms and lipid levels was found in the diabetics, who had a more adverse lipid profile than the selected controls. In the diabetics, but not the controls, in CT carriers compared to CC homozygotes there were lower levels of body mass index (BMI; 23.8 +/- 3.9 vs. 25.4 +/- 4.7 kg/m2, P=0.048) and waist-to-height ratio (0.49 +/- 0.06 vs. 0.52 +/- 0.07, P=0.023), and this relationship was supported by tertile analysis.

CONCLUSIONS

The -75AA genotype was associated with higher HDL-cholesterol levels in the selected healthy, but not diabetic, subjects. The 83T allele was associated with greater indices of obesity in the diabetic patients, and with higher HDL-cholesterol in heterozygous healthy male subjects.

摘要

目的

既往研究报道了两种载脂蛋白A-I（apoA-I）基因MspI多态性（G-75A和C83T）与高密度脂蛋白（HDL）胆固醇和/或apoA-I水平之间的关联，但未研究其与肥胖的关系。

方法

我们测定了482例早发型（≤40岁）2型中国糖尿病患者和167例选取的中国健康对照者中这些多态性的分布情况。

结果

糖尿病患者和健康受试者中-75A和83T等位基因频率相似。在健康对照者中，AA纯合子的HDL胆固醇水平显著高于GG/GA携带者（1.74±0.58 vs. 1.45±0.58 mmol/L，P<0.001）。此外，分析显示在选取的健康受试者中，HDL胆固醇三分位数升高与AA基因型频率之间存在显著关系（3.6%、8.9%和16.1%，P=0.026）。对于C83T多态性，健康男性CT携带者的HDL胆固醇水平高于CC纯合子（1.71±0.57 vs. 1.25±0.30 mmol/L，P=0.001），但在女性中未发现此现象。在糖尿病患者中未发现这些多态性与血脂水平之间的关系，糖尿病患者的血脂谱比选取的对照者更差。在糖尿病患者而非对照者中，与CC纯合子相比，CT携带者的体重指数（BMI；23.8±3.9 vs. 25.4±4.7 kg/m2，P=0.048）和腰高比（0.49±0.06 vs. 0.52±0.07，P=0.023）较低，三分位数分析支持了这种关系。