Choi Ji-Woong, Kim Dong-Jo, Rhim Jung-Hyo, Chung Jun-Ho, Chung Hong-Keun
Department of Biochemistry and Molecular Biology, Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Korea.
Hybrid Hybridomics. 2003 Aug;22(4):259-62. doi: 10.1089/153685903322328983.
Numerous studies have indicated that the oxidative modification of low-density lipoprotein (LDL) plays a critical role in the pathogenesis of atherosclerosis. Malondialdehyde-modified LDL (MDA-LDL) is one of the candidate oxidative products. Therefore, to allow the assessment of oxidized LDL in human serum, we developed monoclonal antibodies for MDA-LDL. Two of these-MDA1 and MDA2-bound to oxidized LDL but not to native LDL by Western blot analysis. The murine monoclonal antibodies to oxidized LDL have potential clinical implications, as imaging agents, for defining the compositions of atherosclerotic vessels in vivo.
大量研究表明，低密度脂蛋白（LDL）的氧化修饰在动脉粥样硬化的发病机制中起关键作用。丙二醛修饰的LDL（MDA-LDL）是候选氧化产物之一。因此，为了能够评估人血清中的氧化LDL，我们开发了针对MDA-LDL的单克隆抗体。通过蛋白质印迹分析，其中两种抗体——MDA1和MDA2——与氧化LDL结合，但不与天然LDL结合。针对氧化LDL的鼠单克隆抗体作为成像剂在体内确定动脉粥样硬化血管的组成方面具有潜在的临床意义。
