Koskela Harri, Kilpeläinen Ilkka, Heikkinen Sami
Department of Chemistry, University of Oulu, PO Box 3000, Oulu FIN-90014, Finland.
J Magn Reson. 2003 Oct;164(2):228-32. doi: 10.1016/s1090-7807(03)00250-7.
A new pulse sequence, long-range CPMG-adjusted heteronuclear single quantum coherence (LR-CAHSQC), is proposed for the determination of long-range JCH coupling constants from a long-range 1H-13C correlation experiment. The long-range heteronuclear coupling constants can be directly extracted from COSY-type antiphase peak patterns. The current approach utilizes CPMG-sequences for polarization transfer, and thus avoids the evolution of homonuclear JHH couplings, which normally may introduce abnormalities into the cross peak pattern. The differences between LR-CAHSQC and normal LR-HSQC are discussed.
提出了一种新的脉冲序列——远程CPMG调整异核单量子相干（LR-CAHSQC），用于通过远程1H-13C相关实验测定远程JCH耦合常数。远程异核耦合常数可直接从COSY型反相峰模式中提取。当前方法利用CPMG序列进行极化转移，从而避免了通常可能给交叉峰模式引入异常的同核JHH耦合的演化。讨论了LR-CAHSQC与常规LR-HSQC之间的差异。
