McCormack J M, Moore S C, Gatewood J W, Walker W S
Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105-2794.
Cell Immunol. 1992 Dec;145(2):359-71. doi: 10.1016/0008-8749(92)90338-p.
A panel of seven mouse splenic macrophage cell lines, derived from cloned progenitors, was compared for their ability to present antigen to Th1 or Th2 helper T cell lines and hybridomas, as well as to naive T cells, and to provide accessory cell function for the synthesis of antibody from primed B cells. One of the cell lines expressed MHC class II molecules and was the only line with constitutive antigen-presenting activity for Th1 cells. It may represent a subset of splenic macrophages responsible for the activation of naive Th1 helper cells in situ. The remaining six cell lines responded to INF-gamma by up-regulating their class II expression and acquiring Th1 antigen-presenting activity. They may represent cells which, in situ, lack constitutive antigen-presenting activity but are promoted to presenting status by Th1-derived INF-gamma. Five of the cell lines provided accessory cell function to Th2 cells, as indicated by antibody synthesis in suspensions of spleen cells from primed mice depleted of their antigen-presenting cells. One of the cell lines lacking accessory cell activity had constitutive antigen-presenting activity for Th1 cells. This reciprocal expression of antigen-presenting activity supports the idea that Th1 and Th2 helper cells are activated by different antigen-presenting cells. Finally, the cell lines differed in their ability to constitutively induce an allogeneic response; a response that was limited to CD8+ T cells occurred in a CD4+ helper cell-independent manner and was unaffected by the addition of INF-gamma. The alloantigen-presenting macrophage cell lines also possessed the most efficient accessory cell activity for antibody synthesis. These cell lines, which represent a spectrum of antigen-presenting activities in the spleen afford models for defining the roles of macrophages in the induction of immune responses and for resolving issues concerning their development.
对一组由克隆祖细胞衍生而来的七种小鼠脾巨噬细胞系进行了比较，以研究它们将抗原呈递给Th1或Th2辅助性T细胞系及杂交瘤、以及呈递给未致敏T细胞的能力，同时研究它们为已致敏B细胞合成抗体提供辅助细胞功能的能力。其中一个细胞系表达MHC II类分子，是唯一对Th1细胞具有组成性抗原呈递活性的细胞系。它可能代表了负责原位激活未致敏Th1辅助性细胞的脾巨噬细胞亚群。其余六个细胞系通过上调其II类表达并获得Th1抗原呈递活性来响应干扰素-γ。它们可能代表了原位缺乏组成性抗原呈递活性但被Th1来源的干扰素-γ促进至呈递状态的细胞。五个细胞系为Th2细胞提供辅助细胞功能，这在已致敏小鼠的脾细胞悬液中去除抗原呈递细胞后的抗体合成中得到了体现。其中一个缺乏辅助细胞活性的细胞系对Th1细胞具有组成性抗原呈递活性。抗原呈递活性的这种相互表达支持了Th1和Th2辅助性细胞由不同抗原呈递细胞激活的观点。最后，这些细胞系在组成性诱导同种异体反应的能力上存在差异；一种仅限于CD8+ T细胞的反应以不依赖CD4+辅助性细胞的方式发生，并且不受干扰素-γ添加的影响。呈递同种异体抗原的巨噬细胞系在抗体合成方面也具有最有效的辅助细胞活性。这些代表了脾中一系列抗原呈递活性的细胞系为定义巨噬细胞在免疫反应诱导中的作用以及解决有关其发育的问题提供了模型。
