川芎嗪对兔阴茎海绵体平滑肌细胞胞浆游离钙浓度的影响

[Effects of tetramethylpyrazine on cytosolic free calcium concentration in penis corpus cavernsum smooth muscle cells in rabbits].

作者信息

Chen Zhi, Liu Ji-Hong, Yin Chun-Ping, Chen Jun, Xiao Heng-Jun

机构信息

Department of Urology, Tongji Hospital, China.

出版信息

Zhonghua Nan Ke Xue. 2003 Aug;9(5):331-4.

PMID:14513636

Abstract

OBJECTIVE

To study the effects of Chinese medicine tetramethylpyrazine (TMP) on intracellular free calcium ([Ca2+]i) in cultured penis corpus cavernosum smooth muscle cell (PCSMC) in rabbits.

METHODS

By using laser scanning confocal microscope (LSCM), the [Ca2+]i fluorescence signal changes was investigated in cultured PCSMC loaded with Ca2+ indicator Fluo-3/AM and divided into potassium chloride(KCl) group and norepinephrine (NE) group. Compared with verapamil (Ver), the effects of TMP was observed in different concentrations on [Ca2+]i increase induced by high potassium and NE.

RESULTS

TMP had no obvious effect on resting PCSMC [Ca2+]i. It was found that 1, 10, 100 mumol/L TMP significantly inhibited [Ca2+]i increase induced by high potassium-depolarization. The peak inhibition rates were (38.6 +/- 3.0)%, (44.1 +/- 2.4)% and (53.7 +/- 4.1)% respectively. TMP could also inhibit cytosolic calcium pool release induced by 1 mumol/L NE. The peak inhibition rates were (13.9 +/- 2.7)%, (21.2 +/- 1.9)% and (29.5 +/- 3.6)% respectively.

CONCLUSIONS

TMP can inhibit rabbit PCSMC [Ca2+]i significantly by suppressing voltage-dependent calcium channel and cytosolic calcium pool release. The effect, similar to Ver, signifies the important mechanism of erectile dysfunction (ED) therapy.

摘要

目的

研究中药川芎嗪（TMP）对兔阴茎海绵体平滑肌细胞（PCSMC）内游离钙（[Ca2+]i）的影响。

方法

运用激光扫描共聚焦显微镜（LSCM），对加载Ca2+指示剂Fluo-3/AM的培养PCSMC进行研究，将其分为氯化钾（KCl）组和去甲肾上腺素（NE）组，观察[Ca2+]i荧光信号变化。与维拉帕米（Ver）比较，观察不同浓度TMP对高钾和NE诱导的[Ca2+]i升高的影响。

结果

TMP对静息PCSMC的[Ca2+]i无明显影响。发现1、10、100μmol/L TMP能显著抑制高钾去极化诱导的[Ca2+]i升高，峰值抑制率分别为（38.6±3.0）%、（44.1±2.4）%和（53.7±4.1）%。TMP还能抑制1μmol/L NE诱导的胞质钙库释放，峰值抑制率分别为（13.9±2.7）%、（21.2±1.9）%和（29.5±3.6）%。