Wilson Joyce A, Richardson Christopher D
Ontario Cancer Institute, 620 University Ave, Suite 706, Toronto, M5G 2C1, Canada.
Curr Opin Mol Ther. 2003 Aug;5(4):389-96.
RNA interference, (RNAi) is a phenomenon by which the introduction of double stranded RNA (dsRNA) into cells induces targeted degradation of RNA molecules with homologous sequences. In the laboratory, RNAi has become a valuable tool for analysis of gene function through suppression of specific gene products. In addition, RNAi has shown great promise for use in therapeutic strategies designed to suppress the expression of pathogenic genes. In this review, viral expression and animal model systems that indicate the potential benefits of RNAi will be discussed. The major obstacle for the use of short interfering RNA in a laboratory or clinical setting is the need for efficient and sustained delivery of dsRNA to mammalian cells. Here, current methods that are being used to induce RNAi, both in cultured cells and in animal models, will be described with a focus on some of the most promising applications.
RNA干扰（RNAi）是一种现象，即向细胞中引入双链RNA（dsRNA）会诱导具有同源序列的RNA分子发生靶向降解。在实验室中，RNAi已成为通过抑制特定基因产物来分析基因功能的宝贵工具。此外，RNAi在旨在抑制致病基因表达的治疗策略中显示出巨大的应用前景。在这篇综述中，将讨论表明RNAi潜在益处的病毒表达和动物模型系统。在实验室或临床环境中使用短干扰RNA的主要障碍是需要将dsRNA高效且持续地递送至哺乳动物细胞。在此，将描述目前在培养细胞和动物模型中用于诱导RNAi的方法，重点关注一些最有前景的应用。
