[血液肿瘤细胞中的寡核苷酸摄取与细胞种类和增殖有关]
[Oligonucleotide uptake in hematological tumor cells is related to cellular species and proliferation].
作者信息
Zhu Yuan-gui, Zhuo Guang-sheng, Chen Zhi-zhe, Chen Xiao-chun
机构信息
Institute of Geriatrics of Fujian Province, Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China.
出版信息
Yao Xue Xue Bao. 2003 Jun;38(6):401-4.
AIM
To explore whether the oligonucleotide uptake in hematological tumor cells is related to cellular species and proliferation.
METHODS
Intracellular mean fluorescence intensity was measured by flow cytometry.
RESULTS
After treatment with FITC-labeled G3139 at the concentration of 0.60 mumol.L-1 for 4 h, the G3139 uptake into peripheral blood mononuclear cell and bone marrow mononuclear cell in hematological tumor patients was significantly higher than that in normal control. There was different uptake of G3139 among the malignant hematological tumor cell strains, and the uptake in cells derived from monocyte, B lymphocyte and myeloid cell was much higher than that in cells derived from T lymphocyte. After treatment with all-trans retinoic acid (ATRA), HL60 cell proliferation was markedly inhibited and the uptake of G3139 decreased significantly.
CONCLUSION
Hematological tumor cells were capable of taking up oligonucleotide, and the oligonucleotide uptake in hematological tumor cells is related to its cellular species and its activation.
目的
探讨血液肿瘤细胞对寡核苷酸的摄取是否与细胞类型及增殖有关。
方法
采用流式细胞术检测细胞内平均荧光强度。
结果
用浓度为0.60μmol·L-1的异硫氰酸荧光素（FITC）标记的G3139处理4小时后，血液肿瘤患者外周血单个核细胞和骨髓单个核细胞对G3139的摄取显著高于正常对照。恶性血液肿瘤细胞株对G3139的摄取存在差异，来源于单核细胞、B淋巴细胞和髓系细胞的细胞对G3139的摄取远高于来源于T淋巴细胞的细胞。用全反式维甲酸（ATRA）处理后，HL60细胞增殖明显受抑，G3139摄取显著降低。
结论
血液肿瘤细胞能够摄取寡核苷酸，血液肿瘤细胞对寡核苷酸的摄取与其细胞类型及其活化有关。
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