泌乳周期中子宫和乳腺中腺苷三磷酸双磷酸酶活性的变化。

Changes in apyrase activity in uterus and mammary gland during the lactogenic cycle.

Valenzuela M A, Collados L, Kettlun A M, Mancilla M, Lara H, Puente J, Aranda E, Chayet L, Alvarez A, Traverso-Cori A

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago.

Comp Biochem Physiol B. 1992 Sep;103(1):113-8. doi: 10.1016/0305-0491(92)90421-m.

Abstract

The purpose of this present research was to explore the possible roles of ATP-diphosphohydrolase (apyrase) in two tissues with high energetic demands during cell proliferation and differentiation. 2. Changes in apyrase activities during the pregnancy lactation cycle were examined in the rat uterus and mammary gland. 3. A significant decrease in apyrase activity (ATPase-ADPase) was observed in the pregnant uterus; this observation correlates with a minor inhibitory effect on platelet aggregation. 4. In mammary gland, the enzyme activity increases during lactation in parallel with an increase in blood supply, synthesis of glycoproteins and cell proliferation. 5. Apyrase activity did not change during the estrous cycle. Estradiol administration to rats slightly increased (20%) both ATPase-ADPase activities. 6. The probable function of apyrase is finally discussed, based on its substrate specificity and subcellular localization.

摘要

本研究的目的是探讨三磷酸腺苷二磷酸水解酶（腺苷三磷酸双磷酸酶）在细胞增殖和分化过程中对两个能量需求较高的组织可能发挥的作用。2. 研究了大鼠子宫和乳腺在妊娠泌乳周期中腺苷三磷酸双磷酸酶活性的变化。3. 在妊娠子宫中观察到腺苷三磷酸双磷酸酶活性（ATP酶 - ADP酶）显著降低；这一观察结果与对血小板聚集的轻微抑制作用相关。4. 在乳腺中，该酶活性在泌乳期间随着血液供应增加、糖蛋白合成和细胞增殖而升高。5. 腺苷三磷酸双磷酸酶活性在发情周期中没有变化。给大鼠注射雌二醇会使ATP酶 - ADP酶活性均略有增加（20%）。6. 基于其底物特异性和亚细胞定位，最终讨论了腺苷三磷酸双磷酸酶的可能功能。