鸡（家鸡）肝脏组织中酰基辅酶A：胆固醇酰基转移酶（ACAT）活性的胞质调节剂。
Cytosolic modulators of acyl-CoA: cholesterol acyltransferase (ACAT) activity in hepatic tissue of the chicken (Gallus domesticus).
作者信息
Greer J L, Hargis P S
机构信息
Department of Poultry Science, Texas Agricultural Experiment Station, Texas A&M University System, College Station 77843-2472.
出版信息
Comp Biochem Physiol B. 1992 Sep;103(1):9-13. doi: 10.1016/0305-0491(92)90405-g.
PMID:1451445
Abstract
- An enzymatic assay for microsomal acyl-CoA: cholesterol acyltransferase (ACAT) activity in chicken hepatic tissues was developed using silicic acid chromatography. 2. ACAT activity was increased in response to liver cytosolic protein preparations suggesting the presence of cytosolic modulators of the enzyme. 3. Fractions containing low molecular weight proteins (12-15 kDa) prepared by molecular sieve chromatography also stimulated ACAT activity. 4. Liver-FABP was specifically implicated as a cytosolic modulator of ACAT activity in chicken liver tissue.
摘要
- 利用硅酸色谱法建立了一种用于检测鸡肝组织微粒体酰基辅酶A：胆固醇酰基转移酶（ACAT）活性的酶促测定法。2. 肝胞质蛋白制剂可使ACAT活性升高，这表明存在该酶的胞质调节剂。3. 通过分子筛色谱法制备的含有低分子量蛋白质（12 - 15 kDa）的组分也能刺激ACAT活性。4. 肝脂肪酸结合蛋白被明确认为是鸡肝组织中ACAT活性的胞质调节剂。
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