人、羊、牛和大鼠脂肪组织匀浆中甘油脂质生物合成的体外比较。

Comparison of glycerolipid biosynthesis in homogenates from human, ovine, bovine and rat adipose tissue in vitro.

Rule D C

Department of Animal Science, University of Wyoming, Laramie, WY 82071.

Comp Biochem Physiol B. 1992 Sep;103(1):93-100. doi: 10.1016/0305-0491(92)90418-q.

Abstract

Assay conditions were compared for glycerolipid biosynthesis in homogenates prepared from human abdominal, ovine and bovine subcutaneous, and rat epididymal adipose tissues. 2. In contrast to other species, longer incubation time and greater homogenate concentration resulted in decreased glycerolipid biosynthesis with rat adipose tissue homogenates. 3. Species differences were observed in concentration-dependency for ATP and fatty acids (palmitate, oleate and palmitoleate). 4. Results indicated that glycerolipid biosynthesis transpired at different rates in the four species, and that ovine and human adipose tissue homogenates had similar properties.

摘要

对从人腹部、绵羊和牛的皮下以及大鼠附睾脂肪组织制备的匀浆中甘油脂质生物合成的测定条件进行了比较。2. 与其他物种不同，较长的孵育时间和较高的匀浆浓度导致大鼠脂肪组织匀浆的甘油脂质生物合成减少。3. 在ATP和脂肪酸（棕榈酸、油酸和棕榈油酸）的浓度依赖性方面观察到物种差异。4. 结果表明，甘油脂质生物合成在这四个物种中的发生速率不同，并且绵羊和人脂肪组织匀浆具有相似的特性。