人核糖核酸酶抑制剂变体在大肠杆菌和家蚕昆虫细胞（Bombyx mori）中的表达

[Expression of a human ribonuclease inhibitor variant in Escherichia coli and silkworm insect cell (Bombyx mori)].

作者信息

Huang Guang-Hua, Yang Guan-Zhen, Chen Jiang-Ye, Wu Xiang-Fu

机构信息

State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.

出版信息

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003 Oct;35(10):960-3.

PMID:14515218

Abstract

Ribonuclease inhibitor (RI) is an acidic 50 kD protein with a high content of leucine and cysteine residues. RI inhibits RNases of the pancreatic type. A variant of RI was cloned from human fetal liver cDNA library by polymerase chain reaction (PCR). Compared with the reported RI, only two variations Arg(359)Ala and Leu(365)Pro were found in RIv amino acid sequence. Recombinant RIv has been expressed both in Escherichia coli and silkworm insect cells (Bombyx mori). The recombinant RIv exhibited inhibition activity on ribonucleolytic activity of RNase A in vitro system.

摘要

核糖核酸酶抑制剂（RI）是一种酸性50kD蛋白，富含亮氨酸和半胱氨酸残基。RI可抑制胰腺型核糖核酸酶。通过聚合酶链反应（PCR）从人胎儿肝脏cDNA文库中克隆出RI的一个变体。与已报道的RI相比，在RIv氨基酸序列中仅发现两个变异，即Arg(359)Ala和Leu(365)Pro。重组RIv已在大肠杆菌和家蚕昆虫细胞（Bombyx mori）中表达。重组RIv在体外系统中对核糖核酸酶A的核糖核酸分解活性表现出抑制活性。