Nascimento-Silva Sheila, Gattass Ricardo, Fiorani Mario, Sousa Aglai P B
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, Rio de Janeiro 21941-900, Brazil.
J Comp Neurol. 2003 Nov 3;466(1):104-18. doi: 10.1002/cne.10878.
Gattass and collaborators (Gattass R, Rosa MGP, Souza APB, Piñon MCG, Neuenschwander S [1990a] Braz J Med Biol Res 23:375-393) proposed that the dorsal stream of visual processing, as defined by Ungerleider and Mishkin (Ungerleider LG, Mishkin M [1982] In: Ingle DJ, Goodale MA, Mansfield RJW, editors. Analysis of visual behavior. Cambridge: Massachusetts Institute of Technology. p 549-586), can be subdivided into dorsolateral and dorsomedial streams, and suggested that they may be involved in different aspects of the processing of motion and spatial perception, respectively. The goal of the present study was to provide additional evidence for this hypothesis by using cytochrome oxidase immunohistochemistry combined with retrograde tracing techniques. In Old World monkeys, the locations of visual area 4 (V4; ventral stream) and middle temporal area (MT; dorsal stream) projecting neurons in V2 supports the hypothesis that the cytochrome oxidase (CytOx)-rich thin stripes and the CytOx-poor interstripes are associated with the ventral stream, and that the CytOx-rich thick stripes belong to the dorsal stream. In this study we describe, in the New World monkey Cebus, the distribution of retrogradely labeled cells in V2 relative to the CytOx compartments after fluorescent tracers were placed in areas V4, MT, and the parietooccipital area (PO). We found PO-projecting neurons in CytOx-rich thick stripes and CytOx-poor interstripes in V2, whereas MT-projecting neurons appeared almost exclusively in thick stripes. In contrast, V4-projecting neurons were located mostly in CytOx-poor interstripes and CytOx-rich thin stripes. In addition, V4- and MT-projecting neurons were located mainly in supragranular layers, whereas PO-projecting neurons were located in supragranular and infragranular layers. These results support the hypothesis for the existence of three distinct streams of visual processing: ventral (including V4), dorsolateral (including MT), and dorsomedial (including PO).
加塔斯及其合作者（加塔斯R、罗莎MGP、苏扎APB、皮尼翁MCG、诺伊恩施万德S [1990a]《巴西医学与生物学研究杂志》23:375 - 393）提出，如昂格莱德和米什金所定义的（昂格莱德LG、米什金M [1982] 载于：英格尔DJ、古德尔MA、曼斯菲尔德RJW编，《视觉行为分析》。剑桥：麻省理工学院。第549 - 586页），视觉处理的背侧通路可细分为背外侧和背内侧通路，并表明它们可能分别参与运动处理和空间感知的不同方面。本研究的目的是通过使用细胞色素氧化酶免疫组织化学结合逆行追踪技术为这一假说提供更多证据。在旧世界猴中，视觉区域4（V4；腹侧通路）和颞中区（MT；背侧通路）在V2中投射神经元的位置支持了这一假说，即富含细胞色素氧化酶（CytOx）的细条纹和细胞色素氧化酶含量低的条纹间带与腹侧通路相关，而富含细胞色素氧化酶的粗条纹属于背侧通路。在本研究中，我们描述了在新世界猴僧帽猴中，在将荧光示踪剂注入V4、MT和顶枕区（PO）后，V2中相对于细胞色素氧化酶区室的逆行标记细胞的分布。我们在V2中富含细胞色素氧化酶的粗条纹和细胞色素氧化酶含量低的条纹间带中发现了投射到PO的神经元，而投射到MT的神经元几乎只出现在粗条纹中。相比之下，投射到V4的神经元大多位于细胞色素氧化酶含量低的条纹间带和富含细胞色素氧化酶的细条纹中。此外，投射到V4和MT的神经元主要位于颗粒上层，而投射到PO的神经元位于颗粒上层和颗粒下层。这些结果支持了存在三种不同视觉处理通路的假说：腹侧（包括V4）、背外侧（包括MT）和背内侧（包括PO）。
