Shekhtman D G, Safronova V G, Gabdulkhakova A G, Miller A V, Sklifas A N, Kantsov V V, Kukushkin N I
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
Biofizika. 2003 Jul-Aug;48(4):666-72.
The ability of the emulsion of perfluoroorganic compounds stabilized with proxanol 268 to affect the functions of peritoneal neutrophils was evaluated. The functional activity of neutrophils was estimated from the intensity of generation of reactive oxygen species using the method of chemiluminescent analysis. The emulsion was shown to suppress the neutrophil responses to phorbol-12-myristate-13-acetate in a dose-dependent manner. No inhibition of the activity of neutrophils in the presence of the emulsion was observed in N-formylmethionylleucylphenylalanine stimulated cells. The data obtained indirectly confirm the suggestion that the perfluoride emulsion inhibits neutrophil NADPH oxidase activity. In the presence of the perfluoride emulsion, myeloperoxidase plays a more important role in the generation of luminescent responses in both N-formylmethionylleucylphenylalanine- and phorbol-12-myristate-13-acetate-stimulated neutrophils. The effect of perfluoride emulsion results in the preferential myeloperoxidase-produced generation of reactive oxygen species in the neutrophil respiratory burst.
评估了用普洛醇268稳定的全氟有机化合物乳液影响腹膜中性粒细胞功能的能力。使用化学发光分析方法，根据活性氧生成强度估计中性粒细胞的功能活性。结果表明，该乳液以剂量依赖的方式抑制中性粒细胞对佛波醇-12-肉豆蔻酸酯-13-乙酸酯的反应。在N-甲酰甲硫氨酰亮氨酰苯丙氨酸刺激的细胞中，未观察到乳液存在时中性粒细胞活性受到抑制。所获得的数据间接证实了全氟化物乳液抑制中性粒细胞NADPH氧化酶活性的推测。在全氟化物乳液存在的情况下，髓过氧化物酶在N-甲酰甲硫氨酰亮氨酰苯丙氨酸和佛波醇-12-肉豆蔻酸酯-13-乙酸酯刺激的中性粒细胞发光反应的产生中发挥更重要的作用。全氟化物乳液的作用导致中性粒细胞呼吸爆发中优先由髓过氧化物酶产生活性氧。
