Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Mech Dev. 2002 Dec;119 Suppl 1:S303-9. doi: 10.1016/s0925-4773(03)00132-1.
Sproutys and Sprouty-related proteins, Spred-1 and -2, are known inhibitors of fibroblast growth factor (FGF) signaling, which plays key role in lung branching morphogenesis and the development of other tissues. The present study demonstrates that Spreds are expressed in a variety of rat embryonic tissues (brain, intestine, heart, skin) including the lung. In the embryonic lung, Spreds and Sproutys are expressed during the early stages of branching morphogenesis, but their expression profiles are both distinct and overlapping. Spreds are predominantly expressed in mesenchymal cells in contrast to Sproutys, which are abundantly expressed in epithelial cells. Spred expression is especially strong in the regions of new bud formation both in the peripheral mesenchyme as well as in the epithelium. The peripheral region also expresses FGF-10 in the mesenchymal cells and FGF-9 in the mesothelial cells. The expression profiles suggest that Spreds, Sproutys and FGF-9/FGF-10 are part of epithelial-mesenchymal interactions, which are essential for the development and maintenance of normal lung branching pattern.
芽殖蛋白和芽殖蛋白相关蛋白（Spred-1 和 -2）是成纤维细胞生长因子（FGF）信号通路的抑制剂，在肺分支形态发生和其他组织发育中起关键作用。本研究表明 Spreds 在多种大鼠胚胎组织（脑、肠、心、皮肤）中表达，包括肺。在胚胎肺中，Spreds 和 Sproutys 在分支形态发生的早期表达，但它们的表达谱既独特又重叠。Spreds 主要在间充质细胞中表达，而 Sproutys 在上皮细胞中大量表达。Spred 的表达在新芽形成的区域特别强烈，无论是在外周间充质还是在上皮中。外周区域的间充质细胞中也表达 FGF-10，间皮细胞中表达 FGF-9。表达谱表明，Spreds、Sproutys 和 FGF-9/FGF-10 是上皮-间充质相互作用的一部分，这对于正常肺分支模式的发育和维持至关重要。
