Ayala Jorge, Dowdy Alan K, Beeman Richard W, Zhu Kun Yan
Department of Entomology, Kansas State University, Manhattan, Kansas 66506-4004, USA.
Arch Insect Biochem Physiol. 2003 Oct;54(2):47-54. doi: 10.1002/arch.10101.
A cDNA encoding subunit Va of cytochrome c oxidase (EC 1.9.3.1) was cloned and characterized from a lesser grain borer (Rhyzopertha dominica) cDNA library. The complete cDNA consists of 693-bp and contains an open reading frame of 450-bp that encodes 150 amino acid residues. The sequence includes a 28-bp putative N-terminal and a 122-bp putative mature protein. The estimated molecular weight and pI for the predicted mature protein are 13,962 and 4.60, respectively. The cDNA-deduced amino acid sequence of the mature protein shows 73% identity to that of a corresponding subunit of African malaria mosquito (Anopheles gambiae) and 59% identity to that of the fruit fly (Drosophila melanogaster). In addition, 31% of all amino acid residues are conserved among six different animal species. Evolutionary distance analysis suggests that cytochrome c oxidase subunit Va from R. dominica is most similar to the corresponding subunit from the malaria mosquito. Northern analysis revealed a single 4.9-kb transcript that is much larger than that found in mammalian species.
从谷蠹（Rhyzopertha dominica）的cDNA文库中克隆并鉴定了编码细胞色素c氧化酶（EC 1.9.3.1）亚基Va的cDNA。完整的cDNA由693个碱基对组成，包含一个450个碱基对的开放阅读框，编码150个氨基酸残基。该序列包括一个28个碱基对的推定N端和一个122个碱基对的推定成熟蛋白。预测成熟蛋白的估计分子量和pI分别为13,962和4.60。成熟蛋白的cDNA推导氨基酸序列与非洲疟蚊（Anopheles gambiae）相应亚基的氨基酸序列具有73%的同一性，与果蝇（Drosophila melanogaster）的相应亚基具有59%的同一性。此外，在六种不同动物物种中，所有氨基酸残基的31%是保守的。进化距离分析表明，谷蠹的细胞色素c氧化酶亚基Va与疟蚊的相应亚基最为相似。Northern分析显示有一个单一的4.9kb转录本，其大小远大于在哺乳动物物种中发现的转录本。
