Uversky V N, Semisotnov G V, Pain R H, Ptitsyn O B
Institute of Protein Research, Russian Academy of Sciences, Moscow.
FEBS Lett. 1992 Dec 7;314(1):89-92. doi: 10.1016/0014-5793(92)81468-2.
The Gdm-HCl-induced unfolding of bovine carbonic anhydrase B and S. aureus beta-lactamase was studied at 4 degrees C by a variety of methods. With the use of FPLC it has been shown that within the transition from the molten globule to the unfolded state the distribution function of molecular dimensions is bimodal. This means that equilibrium intermediates between the molten globule and the unfolded states are absent, i.e. the molten globule unfolding follows the 'all-or-none' mechanism.
在4℃下，通过多种方法研究了盐酸胍诱导的牛碳酸酐酶B和金黄色葡萄球菌β-内酰胺酶的去折叠过程。利用快速蛋白质液相色谱法（FPLC）已表明，在从熔球态向去折叠态转变的过程中，分子尺寸的分布函数是双峰的。这意味着在熔球态和去折叠态之间不存在平衡中间体，即熔球态去折叠遵循“全或无”机制。
