Romanov V A, Kratnov A E, Romanova N V, Khrustalev O A
Patol Fiziol Eksp Ter. 2003 Jul-Sep(3):14-7.
Spectrophotometric NBT-test and luminol-dependent chemiluminescence were used to study oxygen-dependent metabolism of neutrophils and monocytes; antioxidant defense was evaluated by activity of superoxide dismutase measured by spectrophotomety and peroxide chemiluminescence of blood plasma; heparin and trimetasidine (preductal) effects on the above metabolism were studied in 39 patients with unstable angina pectoris. All the patients were found to have a significant rise in functional activity of neutrophils and monocytes by NBT-test and insignificant one by chemiluminescence, unchanged activity of serum superoxide dismutase, reduced total antioxidant potential of blood serum, moderate elevation of circulating immune complexes in high parameters of NBT-test. The latter points to a mild activation of leukocytes through FcR or CR and suggests the presence of another activator of membrane NADP.H-oxidase in unstable angina. Use of heparin in unstable angina activated oxygen-dependent metabolism of neutrophils and monocytes in the absence of reduction in the degree and extent of ischemic myocardial damage. This reduction was observed in the treatment of unstable angina with preductal. This effect is due to suppression, primarily, of NADP.H- and NAD.H-oxidase of leukocytes. It also demonstrates antioxidant properties of this drug.
采用分光光度法NBT试验和鲁米诺依赖的化学发光法研究中性粒细胞和单核细胞的氧依赖代谢；通过分光光度法测定超氧化物歧化酶活性和血浆过氧化物化学发光评估抗氧化防御；在39例不稳定型心绞痛患者中研究肝素和曲美他嗪（冠脉前的）对上述代谢的影响。所有患者通过NBT试验发现中性粒细胞和单核细胞的功能活性显著升高，而通过化学发光法发现升高不显著，血清超氧化物歧化酶活性未改变，血清总抗氧化能力降低，在NBT试验高参数下循环免疫复合物中度升高。后者表明白细胞通过FcR或CR轻度激活，并提示在不稳定型心绞痛中存在膜NADP.H氧化酶的另一种激活剂。在不稳定型心绞痛中使用肝素可激活中性粒细胞和单核细胞的氧依赖代谢，而缺血性心肌损伤的程度和范围并未降低。在用冠脉前药物治疗不稳定型心绞痛时观察到这种降低。这种作用主要是由于抑制了白细胞的NADP.H和NAD.H氧化酶。它还证明了该药物的抗氧化特性。
